The Rylands Library Papyrus P52 is a fragment from a papyrus codex, measuring only 3.5 by 2.5 inches (8.9 cm × 6.4 cm) at its widest, and conserved at the John Rylands University Library Manchester, UK.

The front (recto) contains parts of seven lines from the Gospel of John 18:31–33, in Greek, and the back (verso) contains parts of seven lines from verses 37–38.

Although Rylands P52 is generally accepted as the earliest extant record of a canonical New Testament text, the dating of the papyrus is by no means the subject of consensus among scholars. The original editor proposed a date range of 100–150 CE.